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Additionally, it has been reported that nicotine metabolism is impaired and even reduced by tobacco smoke through the inhibition of CYP2A6, which is mainly responsible for the conversion to cotinine (Lee et al., 1987

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The gas phase extracts of cigarette smoke at the virtual tar concentration of 10 mg/ml PBS were prepared from Hi-Lite brand cigarettes by either continuous (cCSE) or puff smoking protocol (pCSE), and they were subjected to MTS reduction assay (A) and LDH leakage assay (B)

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