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Typically, a dispersion of 5 mg of FA-SNCD@SBA-15/ENZ in 4 mL of phosphate-buffered saline (PBS) with a pH value of 7.4 was prepared and transferred into a dialysis bag with a molecular cutoff of 3.0 kDa

This review integrates mechanistic insights with translational advances, outlining how dysregulation of chaperones, autophagy-mitophagy, the ubiquitinproteasome system, and ER stress pathways drive human diseases, while highlighting emerging therapeutic platforms, from pharmacological chaperones and autophagy modulators to targeted protein degradation technologies, CRISPR screens, spatial biology, and AI-guided drug discovery

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