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We consider comments related to the OPPS payment rate for CPT code 99453 to be out of the scope of the proposed rule

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The transepithelial potential difference was clamped to 0 mV using a DVC 1000 amplifier (WPI, Sarasota, FL, USA) and the resulting short-circuit current was recorded through Ag-AgCl electrodes and 3 mol l 1 KCl agarose bridges

ab104140, dilution ratio 1:200, UK) was then dropped onto the sections and left in the dark for five minutes before a coverslip was placed on top