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The library pool was quantified by real-time PCR using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System and then sequenced on an Illumina NovaSeq6000 sequencing instrument with a paired-end 2 150 bp

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doi: 10.1042/bj1910421 44 ZimmermannMBAeberliI

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250,251,252 UA induced inflammation and relative mechanism Uric acid signaling triggers the activation of several transcription factors, such as NF-B or AP-1, through the activation of MAPK p38 and ERK pathways, resulting in the production of reactive oxygen species (ROS) under the conditions of hyperuricemia