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Epstein MA, Achong BG, Barr YM

Common Mistakes During Reconstitution Using tap water instead of bacteriostatic water Applying vigorous shaking instead of gentle swirling Failing to refrigerate the reconstituted solution Not labeling the vial with concentration or date Over-diluting or under-diluting the peptide solution Each of these errors can lead to inaccurate concentration, reduced peptide stability, or contamination

During measurements, temperature was set and kept constant at 25 C