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According to the model, the carbonyl group of the N-terminal peptide bond of 23 is coordinated to the Zn 2+ ion, whereas the terminal amine is fixed by three glutamate carboxylates (Glu431, Glu487, and Glu295) ( 27 , it was anticipated that the hydroxyl group of the tyrosine side chain of 25 makes a hydrogen-bond with Glu494 of IRAP, an interaction that is lost in the case of 27 according to the free energy perturbation (FEP) analysis ( 27 as compared to the analogous compound 25

These improvements correlate directly with reductions in insulin levels and androgen markers

Biological & Pharmaceutical Bulletin, 44 (12), 18321836

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The use of immunoprecipitation for purification and antigen detection in North America and Europe has become common