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distributed by LGC Standards GmbH, Wesel, Germany) were routinely cultivated in Dulbeccos modified Eagles medium (DMEM)/F-12 (11330057, Thermo Fisher Scientific), supplemented with 10 % FBS and penicillinstreptomycin in a humidified incubator at 37 C with 5% CO 2

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Afterwards, very carefully ring out the excess water and remove any residual stems that prevent it from burning evenly or can make it become harsh

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